PARASITOLOGY - MONSTERS INSIDE ME

INTRODUCTION – WHY YOU SHOULD CARE ABOUT…

Where to start? Parasites are amazing and they have been associated with living creatures and co-evolved and entered an arms-race since the beginning of life on earth! Although you may not think of parasites are very common in the Western world, it is a huge economic problem in livestock, pets and even in humans. Not just in poor countries, but even in the USA, on your doorstep, parasitic infections affect millions of Americans, regardless of socioeconomic status. The five neglected parasitic disease in the US are Chagas disease, cysticercosis, toxocariasis, toxoplasmosis, and trichomoniasis, according to the CDC. Out of this list, my favorite is Toxoplasma gondii, hands down. This mind altering parasite makes enemies love each other (check out Ed Yong's TED talk "Zombie roaches and other parasite tales", minute 9:30 min, talks about Toxoplasma, the parasites changes the behavior of rats to be attracted to cats......by producing dopamine...and can change the behavior of humans, too!)

“Humans are hosts to nearly 300 species of parasitic worms and over 70 species of protozoa, some derived from our primate ancestors and some acquired from the animals we have domesticated or come in contact with during our relatively short history on Earth. Our knowledge of parasitic infections extends into antiquity, and descriptions of parasites and parasitic infections are found in the earliest writings and have been confirmed by the finding of parasites in archaeological material.” (1)

This video is of Trypanosoma brucei, a parasite I studied for my PhD, transmitted by the Tsetse fly and prevalent in sub-Saharan Africa. The parasite lives in the blood and can invade the brain where it causes neurological problems. I studied the evolution and function of a protein that contributed to the molecular arms race between the parasite and their host (2.3). The image depicts a 3D illustration of the Malaria parasite multiplying within a red blood cell being released into the bloodstream.

malaria

Access OpenStax: 4.1 Prokaryote Habitats, Relationships, and Microbiomes, 5.1 Unicellular Eukaryotic Parasites, and 5.2 Parasitic Helminths

The department offers an elective "Human Parasitology" MCB4232 taught by Dr. Kima

LEARNING GOALS

After completing this module, students will be able to:

Recall new vocabulary and definitions that pertain to this module.
Compare and contrast commensal, symbiotic, and pathogenic relationships.
Explain the method of how a fecal float is conducted.
Utilize resources to identify fecal derived parasitic eggs.
Examine the occurrence of a variety of parasitic diseases worldwide.
Recall how mind control is executed by a variety of parasites.
Appreciate the co-evolution of humans and parasites over millions of years.

HISTORY CONNECTION

parasites

Next time you are in Washington DC, visit the Keck building of the National Academy of Sciences and check out the scientific history in the main lobby by Larry Kirkland (free of charge) @ 500 Fifth Street, NW (bottom left, malaria parasite, picture taken by Dr. Oli)

Keck Building, Washington. Malaria parasite as art on the wall

CASE STUDY

Case #1: (picture center) Misty loves running with Dr. Oli in San Felasco state park. A few days after visiting the park, her daughter Maya found 2 ticks on Misty and sent this photo to her mom to ask about the weird red ring about only around one of the ticks. What’s the verdict for Misty?

Case #2: (picture right) Misty completed her annual vet visit without any problems and symptoms, her fecal examination came back negative and all her vitals were OK. The weekend following, Dr Oli noticed some “white rice-like items” on Misty’s fecal matter. What’s wrong with Misty?

VOCAB & VIDEOS & WEBSITES

Take a look at the appropriate module for the vocabulary that is being tested: Microbiology @ UF Lab Terminology Quizlet.

LINKS

Merck Manual: Approach to Parasitic Infections

this is a great overview of the main groups of parasites and main diagnostic methods

One key method of detecting intestinal parasites is the fecal float method, which is performed in the life lab

Fecal float video

The fecal flotation procedure is used routinely to look for various eggs, cysts and ameba, which are indicative of parasitic infections of humans and animals. Many parasites spend a part of their life cycle in the GI (gastrointestinal tract) of their host. Life parasites, their eggs or encysted life forms can be found in the fecal matter.

Examples of commonly found parasites in the feces of pets in Florida are Helminths eggs, which includes hook worms (Ancylostoma spp.), round worms (Ascaris spp) and tapeworms (Dipyliidium). Other parasites often found include Giardia (diplomonads), Cryptosporidium (apicomplexa) and Toxoplasma (apicomplexa) cysts. All these parasites are commonly diagnosed through the fecal flotation method.

To identify your parasite eggs go to Novartis Internal Manual for dogs and Cats

To learn about parasitic diseases check out GIDEON

For life cycle description of parasites go to the CDC homepage on parasites

Fecal float DYI description and video (with Paula Rios Glusberger)

QUIZ

Take the quiz.

ACTIVITIES

FACE-TO-FACE LAB ACTIVITY

Parasitology: Fecal Float Procedure

ASSIGNMENTS/DISCUSSIONS

Complete assignments scheduled.

REAL-WORLD APPLICATION

Monsters inside me (Animal Planet show)
- http://www.animalplanet.com/tv-shows/monsters-inside-me/
MUST WATCH 3 year old with intestinal worms emergency surgery
- http://www.youtube.com/verify_age?next_url=http%3A//www.youtube.com/watch%3Fv%3D3wzCq7Dmc4g
Mosquito Game
- http://www.nobelprize.org/educational/medicine/malaria/about.html
Canine heartworm disease
- http://www.youtube.com/watch?v=djzpi4msUd8
Heartworm extraction surgery
- http://www.youtube.com/watch?v=phCod-0tfkE

ELN REFLECTION

Submit your ELN according to your GTA's instructions.

ABOVE AND BEYOND

Protistology Culture Guide

References

(1) Cox, F. E. (2002). History of human parasitology. Clinical microbiology reviews, 15(4), 595-612.

(2) Oli MW, Cotlin LF, Shiflett AM, Hajduk SL. (2006). Serum resistance-associated protein blocks lysosomal targeting of trypanosome lytic factor in Trypanosoma brucei. Eukaryot Cell;5(1):132-139. doi:10.1128/EC.5.1.132-139.2006

(3) Pays, E., Vanhollebeke, B., Uzureau, P. et al. (2014). The molecular arms race between African trypanosomes and humans. Nat Rev Microbiol 12, 575–584. https://doi.org/10.1038/nrmicro3298

Reading: Bugs in the Brain by Robert Sapolsky. Scientific American 288, 94-97 (March 2003)